Biopolymers and cell. 1989. Volume 5. № 6. 61 - 68

N. V. Rodnin, N. M. Gusak, T. L. Levitina, S. A. Atepalikhina, E. A. Kozlov

INVESTIGATION OF TRYPTIC FRAGMENTS OF MALEYLATED GRAiNUUN OF THE AGROTI'S SCGETUM GRANULOS-IS VIRUS

Summary

    The granulin of the A. segetum granulosis virus was maleylated and subjected to tryptic digestion. The fragments were separated by gel filtration, ion-exchange chromalognap'hy, butanol extraction and high-voltage paper electrophoresis and chromatography. Partial and complete ainino acid sequence of the 23 fragments including 293 amino acid residues was determined. 12 unique fragments comprise 222 residues and account for 90 % of the granulin polypeptide chain.