Biopolymers and cell. 1989. Volume 5. № 6. 46 - 52

Yu. V. Patskovsky, T. P. Voloshchuk, A. I. Potopalsky

DETECTION OF DIRECTIONS OF DNA ALKYLATION WITH ETHYLENEIMINE DERIVATIVES AND ISOLATION OF MODIFIED BASES

Summary

    Alkylation of purine bases with ethyleneimine, monoaziridinediethylphosphate and thio-phosphoamide leads to formation of •1-, 3- and 9-alkylderivatives of adenine, 6-alkyIaini-nopurine and 1-, 7- and 9-alkylderivatives of guaninc. 1- and 7-alkylguanine, 1- and 3-alkyladenine, 6-alkylaminopurine were obtained from the acidic hydrolysates of alkylated DNA. Dependence of the physicochemical properties of alkylated DNA on the direction of purine bases alkylation is discussed.