Biopolymers and cell. 1989. Volume 5. № 5. 64 - 70

Yu. V. Patskovsky. T. P. Voloshchuk, A. I. Potopalsky

SOME PROPERTIES OF THE REACTION BETWEEN POLYNUCLEOTIDES AND THIOPHOSPHAMIDE

Summary

    The alkylation of ribonucleosides and polynucleotides with ethylene imine, monoazindine, diethyl phosphate and antitumour agent thiophosphamide has been shown to change usually nucleobases residues. The preferential centres of the aklylation are nitrogen atoms in the positions 7 of guanosine, 1 of adenosine and 3 of pyrimidine nucleosides. The rate of DNA alkylation increases with a decrease of the ionic strength and pH and depends on the conformation and molecular mass of the molecules.