Biopolymers and cell. 1989. Volume 5. № 5. 44 - 48

A. A. Akhrem, V. P. Egorova, A. S. Egorov, V. I. K.rot, D. Yu. Lando, 2. A. Luka

STUDIES OF DNA INTERACTION WITH PROTEIN CONTAMINANTS

Summary

    DNA isolated from the bovine spleen has been investigated for its interaction with protein contaminants. It is shown that the contaminants are not histones and their binding is determined by the nonionic contacts which are destroyed in the presence of urea and retain with high concentrations of NaCl. The protein contaminants do not change thermostability of DNA and they are distributed irregularly among the macromolecules: less than 10 % of DNA contains more than 70 % of all the proteins.