Biopolymers and cell. 1989. Volume 5. № 4. 52 - 58

V. V. Vlasov, E. M. Ivanovo, Yu. D. Krendelev, I. V. Kutyavin, M. N. Ovander, A. S. Ryte, F. P. Svinarchuk, L. A. Yakubov

SENDAI VIRUS ENVELOPES AND ERYTHROCYTE GHOSTS AS MEMBRANE VEHICLES FOR TRANSPORT OF THE REACTIVE OLIGONUCLEOTIDE DERIVATIVES INTO THE CELLS

Summary

    Poor penetration of oligonucleotide derivatives through mammalian cell membranes interferes with their action as inhibitors of cellular nucleic acid functions. This difficulty can be overcome using reconstituted Sendai virus envelopes and erythrocyte ghosts as membrane carriers of the oligonucleotide derivatives. Fast methods for incorporation of reactive oligonucleotide derivatives into membrane carriers have been developed. In the model experiments membrane carriers incorporating alkylating oligothymidylate derivatives are delivered into ascite carcinoma Krebs 2 cells and it is shown that they are transfered efficiently into cytosol and react with complementary poly (A) sequences of cellular RNAs. Efficiency of delivery of oligonucleotide reagents into cells 10—100 times increases when using the membrane vehicles.