Biopolymers and cell. 1989. Volume 5. № 4. 37 - 45

O. V. Rokhlin, A. Solomon, D. Vasse, A. R. Ibragimov, E. L. Arsenleva, G. T. Bogacheua

CONSTANT DOMAIN EPITOPE EXPRESSION OF HUMAN IMMUNOGLOBULIN L CHAINS OF THE KAPPA AND LAMBDA TYPE IN PROTEINS OF DIFFERENT VARIABLE SUBGROUPS

Summary

    Monoclonal antibodies against three different x-chain constant domain epitopes and three different human Ig A-chain C-domain epitopes have been prepared. Some of the epitopes are expressed both in intact Ig molecules and in isolated L-chains, whereas other epitopes are found only in isolated L-chains. The expression of these epitopes has been studied in 40 various myeloma x-chain preparations belonging to four different variable subgroups, and in 52 X-chain preparations of three V subgroups. The expression level in the above epitope of C domain is shown to depend on the variable subgroups of Z,-chains. This implies the existence of certain interactions between V and C domains promoting the alteration in the level of C domain epitope expression. These studies indicate that interactions of structurally distinct V domains with the C domain probably lead to local conformational changes of the C domain.