Biopolymers and cell. 1989. Volume 5. № 3. 102 - 109

A. A. Krutov, A. Yu. Misharin, V. P. Tsibutsky, T. L. Bushueva, V. R. Babaev, V. S. Repin

INDEPENDENT VISUALIZATION OF RECEPTORS FOR THE NATIVE AND MODIFIED LOW DENSITY LIPOPROTEINS IN CELL CULTURES

Summary

    The method is suggested to prepare cholesteryl esters derivatives containing fluorescent chromophores in acyl chains. The reconstitution of low-density lipoproteins (native and malondialdehyde-treated ones) has been carried out by fluorescent cholesteryl esters. Using the receptor mediated binding of fluorescent lipoproteins to human skin fibro-blasts and mouse macrophages the possibility of independent visualization of receptors is shown by means of fluorescent microscopy using observations with different vawe-lenghts.