Biopolymers and cell. 1989. Volume 5. № 3. 95 - 101

G. A. Kraiasyuk, L. 7.. Jakubov, V. V. Sinitsyn, D. S. Domogatsky, O. V. Rohklin

MONOCLONAL ANTIBODIES FOR ONE-STEP ISOLATION OF HIGHLY PURIFIED UROKINASE

Summary

    Five independent hybridom clones producing monoclonal antibodies with high affinity and specificity for human urokinase are isolated using specially adopted screening tech¬nique. Both inhibiting urokinase activity and noninhibiting antibodies are obtained. They do not crossreact with other serin proteases and some of them have dissociation constants lower than 10 pmol/1. Immunosorbents with these monoclonal antibodies are effective for isolation of urokinase from urine: 2 mg of antibodies attached to 1 ml of sepharose binds 0.5 mg of urokinase.