Biopolymers and cell. 1989. Volume 5. № 3. 88 - 94

A. I. Zinchenko, I. L. Popov, V. N. Barai, I. A. Mikhailopulo

5'-PHOSPHORYLATION OF NATURAL NUCLEOSIDES AND THEIR MODIFIED ANALOGS BY INTACT ERWINIA HERB1COLA CELLS: SPECIFICITY WITH RESPECT TO DONORS AND ACCEPTORS OF PHOSPHATE GROUP

Summary

    Nucleoside-phosphotransferase reaction catalyzed by intact cells of Erwinia herbicola 47/3 has been studied. p-Nitrophenyl phosphate is found to manifest the highest activity among phosphate donors tested. All the natural ribonucleotides and ribose-5-phosphate proved to be highly efficient as well. The activity of ribonucleosides as acceptors of phosphate group decreases in the following order: guanosine>uridine>ribothymidine> >cytidine>adenosine>inosine>xanthosine. 2'-Deoxynucleosides proved to be less efficient phosphate acceptors as compared to the corresponding ribonucleosides. The findings obtained suggest that intact cells of Erw. herbicola 47/3 may be used to produce modified nucleoside-5'-monophosphates in preparative amounts.