Biopolymers and cell. 1989. Volume 5. № 3. 71 - 76

A. V. Bakin, I. N. Shotsky

SELECTIVE FLUORESCENT LABELLING OF THE RNA ENDS TO STUDY ITS MACROMOLECULE STRUCTURE IN THE SOLUTION

Summary

    The method por selective fluorescent labelling of the polynucleotides ends is described. Reactions are performed in water with high yields (90-100 %) followed by no modifi¬cation of the RNA bases. The distances between the ends of oligouridylates of different length in duplex of poly(U) -poly(A) is determined by the singlet-singlet energy transfer method.