Biopolymers and cell. 1989. Volume 5. № 3. 67 - 70

L. L. Ivanov, Z. P. Martinkus, R. R. Stapulionis, L. I. Lukosevicius, A. V. Liekls

DISTRIBUTION OF LEUCYL-tRNA SYNTHETASE ACTIVITY IN POSTRIBOSOMAL EXTRACTS FROM THE RABBIT LIVER AND PIG MYOCARDIUM

Summary

    The distribution of leucyl-tRNA synthetase activity in postribosomal extracts from the rabbit liver and pig myocardium has been investigated both in norm and on the model of protein biosynthesis damage, i. e. myocardial ischemia. Gel chromatography of the postribosomal extracts on Sepharose 6B shows that leucyl-tRNA synthetase activity is distributed among the fractions with molecular weight of 1.82-106 or 0.84-106 (for the rabbit liver) and 0.97-106 or 0.132-106 (for pig myocardium). In the case of myocardial ischemia partial redistribution of leucyl-tRNA synthetase activity between the fractions with different molecular mass is observed in both extracts studied. It is supposed that association-dissociation processes of aminoacyl-tRNA synthetases may participate in re¬gulation of the protein biosynthesis rate in the eukaryotic cells.