Biopolymers and cell. 1989. Volume 5. № 3. 54 - 60

P. G. Chistyakov, M. Abdukayumov, A. G. Venijaminova, S. N. Vladitnirov, D. M. Graifer, S. A. Kazakov, G. G. Karpova

rRNA-PROTEIN INTERACTIONS IN ESCHERICHIA COLI 70S RIBOSOMES AND IN COMPLEX OF INITIATION BY DATA OF CROSS-LINKS WITH BIFUNCTIONAL Pt(II) DERIVATIVE

Summary

    rRNA-protein cross-links in 70S 35S-labelled ribosomes in the free state and within ini¬tiation complex ribosome-AUGU6-fMet-tRNAfMet were investigated using bifunctional cleavable reagent {dichloro [N,N,N',N'-tetrakis- (2-aminoethy!) -1.6-hexamethylendiamine-diplatinum(II)]}dichloride. The sets of proteins cross-linked to 16S and 23S rRNA in free 70S ribosomes and in 70S initiation complex were shown to slightly differ. It was found that one of L7/L12 dimers borders on one of 16S rRNA sites (a distance Sjl.5 nm) both in free ribosomes and in initiation complex.