Biopolymers and cell. 1989. Volume 5. № 3. 43 - 49

N. I. Rechkunova, S. G. Lokhov, Yu. A. Gorbunov, V. V. Zinoviev, Ya. I. Buryanov, E. G. Malygin

STABILITY OF SECONDARY STRUCTURE OF THE OLIGONUCLEOTIDE SUBSTRATES. THE EFFECT OF ECODAM DNA-METHYLASE

Summary

    Oligonucleotide complexes containing various defects in Ecodam methylase recognition site have been investigated for their stability. Partial duplex structure of the single-stran¬ded 20-base long oligonucleotide containing a self-complementary hexanucleotide sequ¬ence is observed only below 5 CC. Other complexes are melting within the narrow tempe¬rature range of 22-31 °C in 30 mM of potassium-phosphate buffer, pH 7.8. Presence of Ecodam methylase results in an increase of complex melting point at least by 5 °C.