Biopolymers and cell. 1989. Volume 5. № 2. 102 - 104

A. D. Yaremchuk, M. A, Tukalo, S. P. Egorova, G. Kh. Matsuka

ISOLATION OF THREONYL-tRNA SYNTHETASE FROM THERMUS THERMOPHILUS

Summary

    A method for isolation of high-purified threonyl-tRNA synthetase from T. thermophllus is described, including ammonium sulfate fractionation, chromatography on DEAE-sepharose, hydroxyapatite, heparine-sepharose and hydrophobic chromatography on Toyopearl HW-65. Yield of the purified enzyme was 9 mg from 1 kg of T. thermophllus cells. The enzyme is a dimer protein (a2 type) with molecular mass of 148 kDa.