Biopolymers and cell. 1989. Volume 5. № 2. 69 - 73, 106

Z. Yu. Tkackuk, L. V. Tkachtik, E. I. Kvasyuk, G. V. Zaitseva, E. N. Kalinichenko, I. A. Mikhailopulo, G. Kh. Matsuka

CHANGES IN FUNCTIONAL PROPERTIES OF RESTRICTION ENZYMES UNDER THE INFLUENCE OF (2'-5') OLIGOADENYLATES IN VITRO

Summary

    It is found that «core» trimers of (2'-5') oligoadenylic acid, viz., (A2'p)2A and-(3'dA2'p)23'dA, exert an influence on functional properties of restriction enzymes EcoRl, BamHl, and Hindlll. The presence of (2'-5') trimers in the reaction mixture containing DNA and the restriction enzyme results in (i) an inhibition of the restriction reaction, (ii) site-specific restriction of DNA >, or (iii) nonspecific hydrolysis of DNA K. In the last case restriction enzymes function as nonspecific DNAses.