Biopolymers and cell. 1989. Volume 5. № 2. 58 - 61

L. N. Neborachko, L. L. Lukash, B. M. Troyanovsky, I. S, Varzanova, T. 1. Buzhievskaya, V. A. Kordium

STUDIES IN POSSIBILITY OF EXPRESSION OF EXOGENIC HUMAN INSULIN GENE IN CULTURED MAMMALIAN FIBROBLASTS

Summary

    Expression of human insulin genomic gene is observed in human and murine fibroblasts. Protein-product (proinsulin or insulin) detected by ELISA is secreted by fibroblasts. The protein-product secreted level achieves its peak on 9th-10th days and may be expressed in 125-160 ng/ml of culture medium. The similar dependence of proinsulin / insulin secretion by human and murine fibroblasts is determined.