Biopolymers and cell. 1989. Volume 5. № 1. 72 - 77

F. Sh. Gizatullin, B. I. Barabanschikov

RESTRICTION ENZYME ANALYSIS AND LOCALIZATION OF BACILLUS SUBTIL1S CHROMOSOMAL FRAGMENT PARTIALLY SUPPRESSING recB recC MUTATIONS OF ESCHERICHIA COLI

Summary

    The original plasmid pKUl partially suppressing recB21 recC22 mutations of E. coli contains a 6.9 kb chromosomal fragment of Bac. subtilis. Using this plasmid an inte-grative vector for Bac. subtilis was constructed. The complementation analysis revealed that the cloned fragment contained a new Bac. subtilis gene, which codes the exonuclease activity of ATP-dependent DNAse and differs from recE5 and recH genes. The new gene, designated as recQ, has been located in glyB region on 75° of the Bac. subtilis genetic map.