Biopolymers and cell. 1989. Volume 5. № 1. 66 - 72

I. K. Komarnitsky, A. M. Samoylov, Yu. Yu. Gleba

RESTRICTION MAPPING OF CHLOROPLAST DNAs OF FIVE NICOTIANA SPECIES

Summary

    Restriction mappings for five Nicotiana species of australian origin — N. eastii, N. occidentalis, N. exigua and american one — N. undulata, N. chinensis have been constructed using Sal Gl, Xho I, Pst 1, Pvu II and Sma I restriction endonucleases. Most of changes found in chloroplast DNA are positioned in the central part of the large single-copy region and are adjacent to inverted repeats. These changes are, probably, due to deletions and/or insertions. On the contrary, the changes in the small single-copy region are mainly caused by point mutations.