Biopolymers and cell. 1988. Volume 4. № 5. 254 - 258

L. P. Buchatsky, O. M. Filenko

CERTAIN PHYSICO-CHEMICAL PROPERTIES OF MOSQUITO DENSONUCLEOSIS VIRUS DNA

Summary

    Mosquito densonucleosis virus (MDV) is isolated from the blood-suckling mosquito Aedes aegyptl and properties of its DNA are characterized. The reaction of formaldehyde with MDV virion and extracted DNA suggests that DNA in situ and in vitro is single-stranded. DNA extracted from MDV has a buoyant density of 1.734 g/cm3 in cesium chloride. The S2o, » was determined in alcaline sucrose gradients to be 9.2, that corres¬ponds to the molecular weight 2.5X106 daltons. Basing on the melting curve and the reaction with formaldehyde the DNA of MDV is believed to be closely related to mem¬bers' of the parvovirus subgroup.