Biopolymers and cell. 1988. Volume 4. № 4. 193 - 196

K. A. Soldatkin, A. P. Potapov, A. P. Soldatkin, A. V. El'skaya

STUDY OF poly(U) AND poly(dT)-DEPENDENT Phe-tRNAphe BINDING TO SOS SUBUNITS OF ESCHERICHIA COLI RIBOSOMES

Summary

    To study the role of the codon sugar-phosphate backbones in the mRNA decoding pro¬cess we have compared the messenger activity of authentic ribo- and deoxyribopolynucle-otides, poly(U) and poly(dT), in the factor-free binding of Phe-tRNA^phe to the SOS subunits of E. coll ribosomes. The template efficiency of poly(U) is much higher than that of poly(dT). Template 2'-hydroxyl groups seem to be important for the binding process at the A site of SOS subunit. The results obtained indicate the co-operative na¬ture of the occupation of the P and A sites of SOS subunits. Neomycin exerted an insignificant effect upon the Phe-tRNA^phe binding. High sensitivity of translation efficiency to neomycin may be due to the translocation stage.