Biopolymers and cell. 1988. Volume 4. № 3. 163 - 167

E. B. Paton, I. V. Kroupskaya, A. N. Zhyvoloup

ORIENTATIONAL EFFECTS CAUSED IN THE pUC PLASMID BY E. COL1 rplJL-rpoBC-OPERON FRAGMENTS

Summary

    A number of recombinant pUC plasmids containing E. coil rpUL-rpoBC-operon fragments were constructed. A rplf gene and PL\O promoter containing 1568 bp EcoRI fragment and rplj gene structural part containing BspRl-A-hagment were found to be oriented in pUC plasmids unidirectionally, in such a way that genes rpU of the inserted fragment and lacZ' of the vector were transcribed in the opposite direction.