Biopolymers and cell. 1988. Volume 4. № 3. 151 - 157

O. V. Bazhenova, I. L. Potokin, B. V. Simarov

OBTAINING OF SPHEROPLASTS IN NODULE BACTERIA OF LUCERNE, CLOVER, BEAN AND PEA

Summary

    A simple universal procedure has been developed for obtaining stable spheroplasts from the fast-growing species of Rhizobium (R. meliloti, R. phaseoli, R. trlfolii, R. legumino-sarum). Spheroplasts were prepared by culturing cells in the osmotically stable medium with a decreased content of calcium cations in the presence of penicillin, followed by treatment with lysozyme. The electron-microscopic analysis of the process of sphero¬plasts formation was performed. The average effectiveness of the cell wall regeneration is 1.4-5.0 %.