Biopolymers and cell. 1988. Volume 4. № 2. 101 - 104

N. V. Borisyuk, G. P. Miroshnichenko

STRUCTURAL ORGANIZATION OF RIBOSOMAL DNA IN Atropa belladonna

Summary

    The rDNA structure of A. belladonna was examined by Southern blot-hybridization of EcoRl- and EcoRV-digests of total nuclear DNA using the maize 32P-rRNA-probes and the 32 P-pUC222 plasmid containing the sequence coding for the 3' end of 25S rRNA and a fragment of non-transcribing spacer from the lemon rDNA. The possible physical maps were tentatively constructed for EcoRl and EcoRV cleavage sites around the 18S and 25S rRNA genes. Two size classes of the repeating units of 9.4 and 10.2 kilobases were found containing the EcoRl cleavage sites in the coding regions for 18S and 25S rRNAs. Both the repeating units found contained only one EcoRV cleavage site.