Biopolymers and cell. 1988. Volume 4. № 1. 20 - 27

Yu. A. Kalambet, E, I. Bur\ova, A. A. Juchkov, V. L. Knorre, A. A. Alexandrov

MEASUREMENT Ob DNA-PROTEIN BINDING CONSTANTS BY MEANS OF CHROMATOGRAPH «MILICHROM» —COMPUTER COMPLEX

Summary

    The method of gel-chromatography is used to determine the constants of DNA-protein binding. The complex consisting of multiwave scanning chromatograph «Milichrom» and computer «Iskra-226» is used as a registering device. The software for this complex allows determining DNA and protein concentrations at any moment. Information on time dependence of DNA and protein concentrations is used to determine the constants of DNA-protein binding. The method is applied to investigate the interaction of RNA-polymerase of E. coll by a number of plasmids and four one-stranded oligodesoxyribonucleotides — ihe fragments of E. coll promoters.