Biopolymers and cell. 1987. Volume 3. № 6. 313 - 317

Ye. E. Yegorov, R. R. Gumeniuk, I. A. Prudovsky, A. V. Zelenin

DNA SYNTHESIS IN THE HYBRIDS OF MACROPHAGES AND VARIOUS CELLS WITH LIMITED PROLIFERATIVE POTENTIAL

Summary

    Macrophages from mouse peritoneal cavites were fused with various cells with the limited capasity to proliferative: human and rat embryo fibroblasts, rat chondrocytes, mouse macrophage precursors. DNA synthesis was studied radioautographically. In any case DNA synthesis was not detected in the macrophage nucleus in heterokaryons. This result and some previous data gave a reason to believe that only proliferatively immortal cells are able to reactivate DNA synthesis after fusion with nondividing differentiated cells. It is possible that this ability is determined by the activity of immortalising oncogenes. No inhibition of DNA synthesis in the nuclei of active cells in heterokaryons was observed. This suggests that the maintenance of macrophagcs in non-proliferating state is not connected with the production of intracellular negative regulators of proliferation.