Biopolymers and cell. 1987. Volume 3. № 6. 289 - 294

V. R. Sagitov, A. Z. Metlitskaya, A. A. Aleksandrov

PREFERENCE OF BspRI RESTRICTASE AS TO THE PROPER SITES ON DNA

Summary

    Relative hydrolysis rates are measured for 15 BspRI sites (GGCC) on pBR322 and pUC19 plasmids and for 4 sites on pAO3 plasmid. Some features of nucleotide sequences in 5'-direction from the site have been found: the sequence CPuT or its part PuT adjacent to the site with high hydrolysis rate; PuPu pair in —1-2 position and/or A in —3 position relative to the boundary of sites with low hydrolysis rate; some combinations of these features, but T always in —1 position for sites with intermediate rates. Relative hydrolysis rates of sites are the same for supercoiled and linear DNAs. An attempt is made to explain these results in terms of peculiarities of local DNA structure near the restriction site.