Biopolymers and cell. 1987. Volume 3. № 5. 257 - 262

D. 1. Balkov, A. E. Rachkov, L. I. Kolchinskaya, N. F. Starodub, A. V. Elskaya, V. K. Lishko

INTERACTION OF IN VITRO SYNTHESIZED CASEIN WITH ARTIFICIAL PHOSPHOLIPID VESICULES

Summary

    The model system is developed to study the incorporation of products of eukaryotic mRNA cell-free translation into lecithin liposomes. It is shown that in the presence of casein mRNA the 14C-labeled product of translation is accumulated inside liposomes. When mRNA for globin, a nonsecretory protein, is translated in this cell-free system, 14C-label is not found in the internal volume of liposomes. The polypeptides extracted from liposomes after their incubation in the system of casein mRNA translation interact specifically with anti-casein antibodies. These data provide evidence that the transfer of synthesizing casein through bilaye-red lipid membrane does not require a specific receptor. Insertion and transfer of this protein occur co-translationally, due to the interaction of «signal peptide» with membrane lipids.