Biopolymers and cell. 1987. Volume 3. № 4. 215 - 220, 223

R. A. Zakharyan

TRANSFER AND TRANSITORY EXPRESSION OF HSV-I THYMIDINE KINASE GENE IN THE COMPOSITION OF RECONSTRUCTED VIRUS-LIKE AND NUCLEOPROTEIN COMPLEXES IN THE LIVER CELLS

Summary

    The thymidine kinase gene (tk) of HSV-I is included into the preformed empty capsid of the polyoma virus and in the composition of the obtained stable artificial polyoma-like particles (PLP) is intravenously injected into rats weighing 30 g. Electrophoretic profile of the induced HSV-I tk activity in the liver cells has been investigated. The Southern hybridization analysis of DNA from the whole cells and purified nuclei of hepatocytes, endothelial and Kupffer cells is carried out to reveal gene tk HSV-I. The results obtained indicate that PLP are more effective than Ca-precipitate and nucleoprotein complexes in tk gene transfer in vivo into the liver cells and in the transitory expression of the HSV-I tk-activity. Glucocorticoids stimulate transfer of tk gene into hepatocytes and inhibit its transfer into endothelial and Kupffer cells of the liver.