Biopolymers and cell. 1987. Volume 3. № 3. 152 - 154

N. I. Rechkunova, V. V. Zinoviev, E. G. Malygin, Yu. A. Gorbunou, S. G. Popov, V. F. Nesterenko, Ya. I. Buriyanov

DIMERIZATION OF ECODAM-METHYLASE INDUCED BY THE OLIGONUCLEOTIDE SUBSTRATE

Summary

    The Ecodam-methylase has been investigated for its interaction with the 20-base oligo-nucleotide duplex containing the enzyme recognition site. An increase of the enzyme molecular weight was detected by the gel-filtration and sucrose density gradient centrifu-gation methods. The maximal value of the molecular complex weight was observed when concentrations of the enzyme and the substrate were equal. The results obtained prove the formation of the dimeric enzyme form in the substrate presence.