Biopolymers and cell. 1987. Volume 3. № 2. 59 - 65

S. F. Beresten, B. I. Rubikaite, V. K. Kazakov

LOCALIZATION OF ANTIGENIC DETERMINANTS OF BEEF TRYPTOPHANYL-tRNA SYNTHETASE REVEALED BY MONOCLONAL ANTIBODIES

Summary

    The method of limited cleavage of tryptophanyl-tRNA synthetase with trypsin and BrCN as well as radioimmunoassay and radioimmunoblotting are used to show that the antigenic determinant for monoclonal antibodies Ami is located within the 5 kDa fragment and for the Am3 — within the 9 kDa fragment, the both are located at the N-termini of the polypeptide chains; the antigenic determinant for Am2 residues is within the 24 kDa fragment located in the middle of tryptophanyl-tRNA synthetase polypeptide chain. BrCN-fragment maintaining the Ami antigenic determinant is purified by HPLC; it contains 22-25 amino acid residues. Tryptic fragments and fragments obtained by cleavage only at arginine residues are immunologically inactive against Ami, Am2 and Am3.