Biopolymers and cell. 1986. Volume 2. № 6. 328 - 334
D. P. Grama, N. N. Mashkovsky
THE DISCOVERY AND STUDY OF SUBGENOMIC RNA OF POTATO VIRUS X
Summary
Virus-specific, low-molecular weight RNA (m. m. 0.3-106) is detected in Datura plants infected by potato virus X, using 32P-cDNA synthesized on PVX RNA. The low-molecular weight RNA is observed in infected plants three days after infection and it sediments in the linear sucrose gradient between 18S and 5S by ribosomal RNAs, has a nucleotide sequence to 3'-end of genome RNA. It translates in cell-free system into protein with electrophoretic mobility identical to that of coat protein, i. e. it is subgeno-mic PVX RNA for synthesis of virus coat protein.