Biopolymers and cell. 1986. Volume 2. № 6. 317 - 322
Yu. F. Drygin, K. L. Zuklys
SUPERCOILED DNA AS A SUBSTRATE FOR DETECTION OF ENDONUCLEOLYTIC ACTIVITIES IN COMMERCIAL AND LABORATORY BIOCHEMICAL PREPARATIONS
Summary
Supercoiled DNA is known to be a sensitive substrate for detecting the endonucleolytic activity. Supercoiled pBR322 and other Co/£7-related plasmids are shown to be highly useful both for detecting nonspecific endonucleolytic admixtures in many biochemical preparations and for comparative estimation of certain DNAses and RNAses activity.