Biopolymers and cell. 1986. Volume 2. № 6. 307 - 311
N. G. Filimonov, A. 0. Agashkin, O. D. Sablina, M. A. Aitkhozhln
ISOLATION OF BIOLOGICALLY ACTIVE POLY(A)+ AND POLY (A)- mRNA FROM GERMINATING WHEAT EMBRYOS
Summary
Biologically active poly (A) - mRNA were isolated from polyribosomes and free cytoplasmic informosomes of germinating wheat embryos by benzoylated cellulose chromato-graphy. Purified fractions of poly (A) ~ mRNA of polyribosomes and free isoformosomes do not essentially differ from cytoplasmic poly (A) + mRNA by molecular-weight characteristics and have translational activity in the cell-free system of protein synthesis. It is shown that poly (A) - mRNA of polyribosomes and free informosomes equally stimulate incorporation of [35S]methionine into acidinsoluble product, but the efficiency of their translation is much lower than that of poly (A)+ mRNA. The difference in the translation efficiency of cytoplasmic poly (A) + and poly (A) - mRNA of wheat embryos is obviously the metabolitic peculiarity of these mRNA and is not related to their isolation.