Biopolymers and cell. 1986. Volume 2. 2. 69 - 73

 

V. M. Kochkina

 

CATALYTIC ACTIVITY OF ASPARTATE AMINOTRANSFERASE MICROCRYSTALS

 

Summary

 

    The enzymic activity of crystalline cytosolic aspartate aminotransferase (L.-aspartate: aminotransferase 2-oxoglutarate, EC 2.6.1.1) was determined in suspensions of micro-crystals in 50 % ammonium sulphate and 20 % (wt/vol) polyethylene glycol. The crystals (1-2 [j,m wide) were small enough to preclude diffusional rate limitation. The catalytic activity of the crystalline enzyme is 32 % and 40 % of the enzyme activity in solution, respectively.

 

Summary in Russian