Biopolymers and cell. 1985. Volume 1. № 6. 283 - 292

V. L. Knorre, L. K. Savinkova, R. I. Salganik

OLIGONUCLEOTIDE SEQUENCES SELECTIVELY BOUND BY ESCHERICHIA COLI RNA POLYMERASE

Summary

    It is found that E. coli RNA polymerase selectively binds certain oligonucleotides from the random isoplith mixtures obtained by nuclease digestion of either DNA or RNA. The binding is realized only by catalytically active enzyme under conditions optimal for transcription. Using syntetic oligodeoxyribonucleotides of definite sequences is shown that the efficient binding occurs with oligonucleotides related to the «—10» promoter region comprising the Pribnow box and downstream flankng nucleotides up to the tran¬scription initiation site. Oligonucleotides provided with 5'-terminal aklylating groups are covalently bound to RNA polymerase, a-subunit being labelled with deoxyribonucleotide derivative and (5,p'-subunits — with ribooligonucleotide ones.