Biopolymers and cell. 1985. Volume 1. № 5. 271 - 276

L. F. Dyachenko, Yu. M. Sivolap

THE USE OF THE OKAYAMA-BERG VECTOR SYSTEM PRINCIPLE FOR BARLEY cDNA CLONING

Summary

    A new approach is stated for obtaining full size cDNA molecules which is based on the Okayama-Berg cloning technique. The properties of poly/A/+mRNA from barley endosperms and scheme of obtaining cDNA are described. This cDNA was inserted in plasmid and cloned. Transformation allowed selecting about 2x10³ clones.