Biopolymers and cell. 1985. Volume 1. № 5. 267 - 270

V. N. Podust, G. A. Nevinsky, 0. I. Lavrik

MODIFICATION OF E. COLI PHENYLALANYL-tRNA SYNTHETASE BY l,N6-ETHENOADENOSINE-5'-TRIPHOSPHATE

Summary

    The irradiation of phenylalanyl-tRNA synthetase by nitrogen laser light (X337 nm) in the presence of e ATP results in enzyme inactivation and covalent attachement of nucleotide to the protein. Dependence of the enzyme photoinactivation rate on the eATP concentration is of saturated character. ATP partially protects the enzyme against covalent attachement of eATP. However none of the specific ligands (ATP, Phe, phenylalanylade-nylate) prevents the enzyme inactivation.