Biopolymers and cell. 1985. Volume 1. № 4. 219 - 224

A. S. Raschinkina, N. A. Troitsky, L. A. Okulich

INTRODUCTION OF MU BACTERIOPHAGE INTO AZOTOBACTER CHROOCOCCUM AND INTERGENERIC RP4 :: Mu PLASMID-MEDIATED TRANSFER OF GENES

Summary

    Recombinant plasmid of RP4:: Mu cts62 is constructed. The plasmid is transferred by conjugation with the 10~3-10~4 frequency from E. coli to taxonomically nonrelated bacterium of Azotobactcr chroococcum. Azotobacter strains are revealed where Mu cts62 phage DNA was replicated and about 105 plaque-forming units per ml^-1 was produced. The strain of A.chroococcum 31/8 (RP4 :: Mu cts62) is used as a donor of chromosomal genes in intergeneric mating with E. coli AB1157 recipient. The RP4 :: Mu cts62 plasmid-mediated transfer of thr and his markers is found.