Biopolymers and cell. 1985. Volume 1. № 3. 156 - 158
Z. M. Aleksieva, T. N. Shevchenko, S. S. Malyuta
A STUDY OF LYSINE OPERON ORGANIZATION IN BACILLUS SUBTIL1S
Summary
The pBR322 plasmid was used for constructing pLBl plasmid carrying genes of Bacillus subtills lysine biosynthesis. B. subtilis and E. coli strains (lysine-auxotrophs) are trans¬formed. The plasmid is shown to complement both mutations in seven genes of lysine biosynthesis in E. coli cells and four mutations in B. subtills. The size of DNA insertion,. according to the elctrophoresis data, is 4,5-106.