Biopolymers and cell. 1985. Volume 1. № 3. 154 - 156
L. L. Ivanov, R. R. Stapulionis, L. J. Lukosevicius
PURIFICATION AND PROPERTIES OF LEUCYL-tRNA-SYNTHETASE FROM THE MAMMALIAN TISSUES
Summary
The method of purification of leucyl-tRNA-synthetase (LeuRS) from rabbit liver and pig myocardium is described. Two forms (Ei and £2) of LeuRS are distinguished by hydro-xylapatite chromatography of partially purified enzyme from pig myocardium. The mo¬lecular mass was calculated to be 185000 for LeuRS from rabbit liver and 158000 for EI from pig myocardium. E2 is stated to contain aminoacyl-tRNA synthetase activities of other amino acid specificities and is a complex of enzymes with molecular mass of about 800000.