Biopolymers and cell. 1985. Volume 1. № 1. 14 - 20
V. P. Korzh, T. A. Burakova, A. A. Neyfakh, A. L. Mazin
ENHANCEMENT OF GENETIC APPARATUS OF TELEOST FISH EMBRYOS AFTER snRNA MICROINJECTION
Summary
Amount of snRNA was increased artificially by microinjection into developing embryos of teleost fishes. Radiolabelled snRNA after microinjection into loach embryo is concentrated in cell nuclei. Transport of nonhistone proteins into nuclei increases simultaneously. snRNA injection enhances the incorporation of radiolabelled precursors into DNA (late blastula), RNA (late blastula—gastrula), proteins (late blastula—gastrula—early organogenesis). Such effects are characteristic of snRNA from teleost fishes, insects, amphibia and mammalia. Experiments with trout embryos support these results. The data obtained show the important role of snRNA in embryo genome activity regulation at early embryogenesis of teleost fishes.